Methionine supplementing effects on intestine, liver and uterus morphology, and on positivity and expression of Calbindin-D28k and TRPV6 epithelial calcium carriers in laying quail in thermoneutral conditions and under thermal stress

This study aimed to provide the performance, localization and expression of epithelial calcium transporter channels Calbindin-D28k (Calb) TRPV6, morphology digestive reproductive system laying quail under heat stress (HS), with methionine supplementation (MS). characterized positivity (immunohistochemistry) (real-time PCR) in kidneys, intestine uterus 504 quails different MS (100, 110 120%) temperatures (20, 24, 28 32°C). The animals HS (32°C) had lower villus height, villus:crypt ratio, goblet cell index duodenum jejunum, fewer secondary tertiary uterine folds, smaller hepatic steatosis, increased number distal convoluted renal tubules (CT) positive Calb, proximal CTs. Deleterious effects were minimized for: duodenal crypts, cells decreased Calb intestines kidney, TRPV6 gene kidney (P≤0.05). Epithelial transporters altered due less need for absorption reabsorption more available MS, increasing egg production quality termoneutrality further intestinal area increase (P≤0.001) thermoneutrality. It was concluded that use (120%) is justifiable order partially reverse deleterious on production, carriers, digestory quail.